Analysis of Endoplasmic Reticulum (ER) Stress Induced during Somatic Cell Nuclear Transfer (SCNT) Process in Porcine SCNT Embryos
نویسندگان
چکیده
منابع مشابه
Proteomics Analysis of Lungs from Somatic Cell Nuclear Transfer (SCNT) Bama Miniature Pig (Sus scrofa)
With the advent of transgenic pigs using the somatic cell nuclear transfer (SCNT), SCNT pig was thought to hold great promise in basic research, as well as in agricultural and biomedical applications, including xenotransplantation [1,2]. However, SCNT in pigs is limited by substantial problems with the current technologies, including extremely low efficiency, early embryonic and fetal mortality...
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Hairless mice have been widely applied in skin-related researches, while hairless pigs will be a useful model for skin-related study and other biomedical researches. Dickkopf-related protein 1 (DKK1) is inhibitor of Wnt signaling pathway. Transgenic mice expressing DKK1 transgene under control of a human keratin 14 (K14) promoter display hairless phenotype, which encouraged us to generate trans...
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In various animal species, the main cause of pregnancy loss in conceptuses obtained by somatic cell nuclear transfer (SCNT) are placental abnormalities. Most abnormalities described in SCNT pregnancies (such as placentomegaly, reduced vascularisation, hypoplasia of trophoblastic epithelium) suggest that placental cell degeneration may be triggered by mitochondrial failure. We hypothesized that ...
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SETD2 (SET domain containing protein 2) acts as a histone H3 lysine 36 (H3K36)-specific methyltransferase and may play important roles in active gene transcription in human cells. However, its expression and role in porcine oocytes and preimplantation embryos are not well understood. Here, we used immunofluorescence and laser scanning confocal microscopy to examine SETD2 expression in porcine f...
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Somatic cell nuclear transfer (SCNT or cloning) returns a differentiated cell to a totipotent status; a process termed nuclear reprogramming. Reproductive cloning has potential applications in both agriculture and biomedicine, but is limited by low efficiency. To understand the deficiencies of nuclear reprogramming, our research has focused on both candidate genes and global gene expression pat...
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ژورنال
عنوان ژورنال: Development & Reproduction
سال: 2018
ISSN: 2465-9525,2465-9541
DOI: 10.12717/dr.2018.22.1.073